High Performance Liquid Chromatography Basics

About the Course

This course will help you gain a sound working knowledge of basic, high-performance liquid chromatographic principles and develop a greater understanding of theoretical and practical relationships.

Build the expertise you need to choose optimized column parameters, detector selection, and separation mechanism for the examination of complex mixtures, and improve your on-the-job performance.

  • HPLC theory and best practices
  • Column optimization
  • Detector selection
  • Improve separation of complex mixtures

What You Will Learn

  • Move beyond standard operating procedures, and gain a solid working knowledge of HPLC principles and methods.
  • Build expertise that will help you choose the right column, conditions, detector, and separation mechanism to separate difficult mixtures.
  • Improve your on-the-job performance and skill set.

Who Should Attend

Ideal for R&D scientists and technicians or anyone who uses HPLC and wishes to have a solid theoretical and practical background in this technique.

Course Outline

Module 1 – Introduction

  • Introduction to HPLC
  • Separation Mechanisms
  • Components of HPLC Instrumentation
  • Commercial HPLC Instruments

Module 2 – Theory of HPLC

  • Selection of Separation Mechanism
  • Features of HPLC Chromatograms
  • Resolution of HPLC Columns
  • Resolution vs. Selectivity

Module 3 – Instrumentation

  • Pumps
  • Columns
  • Detectors
  • Ultraviolet
  • Fluorescence
  • Mass Spectrometry
  • Refractive Index
  • Evaporative Light Scattering

Module 4 – Separation Mechanisms

  • Adsorption
  • Partition
  • Normal Phase
  • Reverse Phase
  • Anionic and Cationic Ion Exchange
  • Steric Exclusion
  • Affinity
 Module 5 – Qualitative Analysis
  • Retention Time
  • Retention Indices
  • Ultraviolet Spectroscopy
  • Infrared Spectroscopy
  • Mass Spectroscopy

Module 6 – Quantitative Analysis

  • Integration Methods
  • Area Percent
  • Use of Response Factors
  • Internal Standard
  • External Calibration
  • Method of Additions

Module 7 – Alternate and Optimization Techniques

  • Enhanced UV Detection
  • Capillary Zone Electrophoresis
  • Octanol / Water Partition Coefficients
  • Gradient Optimization
  • Methods Development Criteria
  • Computer Based Optimization

About the Instructor(s)


Dennis Anderson

 

Dennis Anderson retired from Akzo Nobel Surfactants America following a 40+-year career as an industrial analytical chemist.

Cost per Module: $199.00

 

Copyright © [2022] American Chemical Society (ACS Professional Education). All rights reserved.

For permission to reuse content from ACS Professional Education, please contact proed@acs.org.

High Performance Liquid Chromatography Basics Module 1

Module 1 – Introduction

  • Introduction to HPLC
  • Separation Mechanisms
  • Components of HPLC Instrumentation
  • Commercial HPLC Instruments


High Performance Liquid Chromatography Basics Module 2

Module 2 – Theory of HPLC

  • Selection of Separation Mechanism
  • Features of HPLC Chromatograms
  • Resolution of HPLC Columns
  • Resolution vs. Selectivity


High Performance Liquid Chromatography Basics Module 3

Module 3 – Instrumentation

  • Pumps
  • Columns
  • Detectors
  • Ultraviolet
  • Fluorescence
  • Mass Spectrometry
  • Refractive Index
  • Evaporative Light Scattering


High Performance Liquid Chromatography Basics Module 4

Module 4 – Separation Mechanisms

  • Adsorption
  • Partition
  • Normal Phase
  • Reverse Phase
  • Anionic and Cationic Ion Exchange
  • Steric Exclusion
  • Affinity


High Performance Liquid Chromatography Basics Module 5

Module 5 – Qualitative Analysis

  • Retention Time
  • Retention Indices
  • Ultraviolet Spectroscopy
  • Infrared Spectroscopy
  • Mass Spectroscopy


High Performance Liquid Chromatography Basics Module 6

Module 6 – Quantitative Analysis

  • Integration Methods
  • Area Percent
  • Use of Response Factors
  • Internal Standard
  • External Calibration
  • Method of Additions


High Performance Liquid Chromatography Basics Module 7

Module 7 – Alternate and Optimization Techniques

  • Enhanced UV Detection
  • Capillary Zone Electrophoresis
  • Octanol / Water Partition Coefficients
  • Gradient Optimization
  • Methods Development Criteria
  • Computer Based Optimization